Development of nested PCR based on the ViaB sequence to detect Salmonella typhi
نویسندگان
چکیده
منابع مشابه
Detection of Salmonella typhi by nested PCR based on the ViaB sequence.
By using nested PCR based on the nucleotide sequence encoding the Vi antigen, the ViaB region of Salmonella typhi, it has been possible to detect Salmonella typhi at the single-cell level. Amplification with two primer sets, R1, which amplified 599 bp of DNA (nucleotides 745 to 1343), and R2, which amplified 307 bp (nucleotides 877 to 1183), was associated with highly specific results for S. ty...
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15 صفحه اولThe Vi antigen of Salmonella typhi: molecular analysis of the viaB locus.
Strains of Salmonella typhi isolated from the blood of patients with typhoid fever invariably express a capsular polysaccharide, termed the Vi antigen. Vi antigen expression is controlled by two separate chromosomal loci, viaA and viaB. The viaA locus is commonly found in enteric bacteria. In contrast, the viaB locus appears to be specific to Vi-expressing strains of Salmonella and Citrobacter....
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1995
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.33.11.3082-3082.1995